Capto ImpRes media are based on a high-flow agarose base matrix with good pressure/flow properties (Fig 3) and a small bead size (approx. The strong ion exchange ligand groups of Capto SP ImpRes (left) and Capto Q ImpRes (right) are well established in large-scale purifications. The ligand of Capto SP ImpRes is a sulfonate group while the ligand of Capto Q ImpRes is a quaternary amine group (Fig 2). Chromatography media characteristics Ionic groups and base matrix Capto SP ImpRes and Capto Q ImpRes are strong cation and strong anion exchangers, respectively. Capto SP ImpRes and Capto Q ImpRes extend the well-established Capto platform to include high-resolution media for late intermediate purification and polishing. Security of supply and comprehensive regulatory support Fig 1.Higher manufacturing productivity enables improved process economy.High-throughput purifications easy to optimize and scale up.Flexible process design due to large operational window of flow rates and bed heights.High-resolution intermediate purification and polishing based on the well-established Capto platform with traditional ligands.Key benefits of Capto SP ImpRes and Capto Q ImpRes include: Based on the well-established Capto base matrix and with traditional ionic group ligands, they provide many opportunities for improved productivity and straightforward process development. Capto SP ImpRes and Capto Q ImpRes are BioProcess chromatography media developed for large-scale purification of biopharmaceutical proteins. The ability to run at higher flow rates and higher bed heights also increases flexibility in process design. By combining the high-flow characteristics of Capto media with a small particle size, Capto SP ImpRes and Capto Q ImpRes deliver excellent pressure-flow properties with impressive resolution. Both chromatography media (resins) are part of an expanded high-resolution platform based on the high-flow agarose Capto product line. Linear gradient elution in the linear range of the adsorption isotherm could successfully separate A1 and A2 and was evaluated for the characteristic charge and the equilibrium constant that were generated Batch adsorption experiments using a robotic workstation for high throughput and accuracy provided non-linear adsorption isotherms and the steric factor.GE Healthcare Life Sciences Data file 28-9837-63 AB Ion exchange chromatography Capto SP ImpRes Capto Q ImpRes Capto SP ImpRes and Capto Q ImpRes are strong cation and strong anion exchangers for the high-throughput intermediate purification and polishing steps of a wide range of biomolecules. The experiments necessary to determine the three SMA parameters were chosen in a way to limit the amount of peptides needed. In ion-exchange chromatography, the adsorption equilibrium between salt and proteins can be described using the steric mass action (SMA) formalism.In this study, the antiviral lantipeptides labyrinthopeptin A1 and A2, purified from Actinomadura namibiensis culture broth, were characterized for their adsorption behavior in anion-exchange chromatography in the range from pH 5.0-7.4. One important compound of such a model is the adsorption behavior of the components of interest. Model-based development for chromatographic separations has proven to be a useful tool for developing reliable purification processes. However, the availability of most lantipeptides is low, and systematic approaches for downstream processing of this group of peptides is still lacking. Lantipeptides from bacterial sources are increasingly important as biopharmaceuticals because of their broad range of applications.
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